摘要
α-Amylase from Aspergillus oryzae was purified by gel filtration and ion-exchange chromatography. It appeared to be homogeneous by ultracentrifugation, electrophoresis, and ion-exchange chromatography, and contained glucosamine, mannose, galactose, arabinose, and xylose. Exhaustive proteolysis of the amylase yielded Ser · Asp(NH · Oligosaccharide) and Asp(NH · Oligosaccharide). By chromatography on Dowex 50-X2, the latter glycopeptide could be fractionated into several peaks, each having a different sugar composition. This implies a heterogeneity of the carbohydrate group of the α-amylase. © 1969.