Abstract
Low-cost paper-based diagnostic device for human sperm motility has developed to know sperm motility without consulting fertility clinics. We compared the signal pattern of the paper-based sperm motility assay using 4-channel pattern (cross) and 2-channel pattern (line) papers, and investigated difference in signal patterns using some tetrazorium salts for the assay. Using these two paper patterns, we can examine whether human sperm motility in semen is more than 50% or 0%. XTT and WST-8 reagents treated paper-based device can be used. We concluded that MTT treated line pattern paper is suitable for the purpose to reduce the cost of the device.