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Photochemical cleavage of single- and double-stranded oligonucleotides by 3-(p-tolylamino)-1,5-azulenequinone
Journal article   Peer reviewed

Photochemical cleavage of single- and double-stranded oligonucleotides by 3-(p-tolylamino)-1,5-azulenequinone

Fu-Yuan Tsai, Shwu-Bin Lin, Shwu-Chen Tsay, Wei-Chen Lin, Chia-Lin Hsieh, Shih Hsien Chuang, Lou-Sing Kan and Jih Ru Hwu
Tetrahedron Letters, Vol.42(33), pp.5733-5735
13/08/2001

Abstract

Azulenequinone Deoxyguanosine DNA cleavage Photochemistry Site-specificity
Irradiation, with 350 nm UV light, of specially designed and synthesized single- and double-stranded oligodeoxyribonucleotides in the presence of 3-(p-tolylamino)-1,5-azulenequinone produced fragments resulting from the cleavage at the deoxyguanosine residue only. The cleaving efficiency was greater for a single strand than a double helix. The efficiency was increased for a less stable double helix, or while the deoxyguanosine residue therein was located at a bulge, at a hairpin loop, or towards the end of the helix. © 2001 Elsevier Science Ltd. All rights reserved.

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