摘要
We previously developed chicken interleukin-1 beta (IL-1 beta) mutants as single-dose adjuvants that induce protective immunity when co-administered with an avian vaccine. However, livestock such as pigs may require a vaccine adjuvant delivery system that provides long-lasting protection to reduce the need for successive booster doses. Therefore, we developed chitosan-coated alginate microparticles as a carrier for bovine serum albumin (BSA) or porcine IL-1 beta (pIL-1 beta) and assessed their physical, chemical, and biological properties. Electrospraying of the BSA-loaded alginate microparticles (BSA/ALG MPs) resulted in an encapsulation efficiency of 50%, and those MPs were then coated with chitosan (BSA/ALG/CHI MPs). Optical and scanning electron microscopy, zeta potential analysis, and Fourier transform infrared spectroscopy were used to characterize these MPs. The BSA encapsulation parameters were applied to ALG/CHI MPs loaded with pIL-1 beta, which were not cytotoxic to porcine fibroblasts but had enhanced bio-activity over unencapsulated pIL-1 beta. The chitosan layer of the BSA/ALG/CHI MPs prevented burst release and facilitated sustained release of pIL-1 beta for at least 28 days. In conclusion, BSA/ALG/CHI MPs prepared as a carrier for pIL-1 beta may be used as an adjuvant for the formulation of pig vaccines.