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Quantitative Attachment and Detachment of Bacterial Spores from Fine Wires through Pulsed Voltammetry
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Quantitative Attachment and Detachment of Bacterial Spores from Fine Wires through Pulsed Voltammetry

Wenbo Zhou, Sarah K. Watt, De-Hao Tsai, Vincent T. LeeMichael R. Zachariah
The Journal of Physical Chemistry B The Journal of Physical Chemistry B, 卷.117(6), 頁碼.1738-1745
2013

摘要

We demonstrate the uniform attachment of bacterial spores electrophoretically onto fine wires in liquids and subsequently quantitatively detached back into suspension. It was found that the use of a pulsed voltage method resulted in a uniform coverage of spores and prevented visible bubble formation resulting from water electrolysis which tended to dislodge the spores from the wires. By monitoring the electrophoretically derived current, this method could also be used to quantitatively measure the surface charges on spores and the deposition rate. The method is generic and should be applicable to the deposition of any charged biological material (e.g., spores, bacteria, viruses) onto metal surfaces.

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