摘要
We propose a new and highly effective tool to add to the ever shrinking toolbox for combating infections caused by antibiotic resistant bacteria; N-chloramine and quaternized N-chloramine were coated onto iron-oxide magnetic nanoparticles to generate antibacterial MNPs. Two differently-sized primary iron-oxide nanoparticles (3 nm and 10 nm) were synthesized and coated with silica and (3-chloropropyl)triethoxysilane, allowing subsequent introduction of N-chloramine precursors-dimethyl hydantoin (DMH) and quaternized dimethyl hydantoin (QDMH). The functionalized MNPs (MNP@DMH and MNP@QDMH) have a clear core-shell structure as evidenced by TEM images. Fe <sub>3</sub> O <sub>4</sub> was identified (by combining X-ray diffraction with Mössbauer spectroscopy) to be the iron oxide in the 10 nm MNPs, while γ-Fe <sub>2</sub> O <sub>3</sub> and Fe <sub>3</sub> O <sub>4</sub> were the 3 nm MNP's oxide phases. Both MNPs (3 nm and 10 nm) have good magnetic responses, with saturation magnetizations of 40 ± 4 emu g <sup>-1</sup> and 65 ± 2 emu g <sup>-1</sup> , respectively. Chlorination activated the antibacterial function and yielded two antibacterial MNPs: MNP@DMCl and MNP@QASCl. At the equivalent [Cl <sup>+</sup> ] of 50 ppm, both coatings demonstrated fast inactivation of the model bacteria methicilin-resistant Staphylococcus aureus (MRSA) and multi-drug resistant (MDR) Pseudomonas aeruginosa. For either size of primary MNPs, MNP@QDMCl is more effective than MNP@DMCl. A hand-held magnet could quickly remove >99% of the functionalized MNPs from a wound simulant within 2 minutes.