Abstract
Selenium concentration in serum has been proposed as the most valuable indicator of human selenium status. In this work, the concentration of selenium in serum is measured by GFAAS using longitudinal Zeeman-effect background correction, a highly sensitive technique that can be used to determine selenium at sub-ppb level. Standard reference materials are analyzed to verify the accuracy and precision of this technique. The direct method proposed in this study is used for the determination of selenium in human serum collected from healthy people of 19-25 years and women of 21-34 years in the first trimester of pregnancy. The method developed in this study, which can quickly and accurately measure serum selenium, is suitable for applications in clinical diagnosis or biological monitoring.