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Series quartz crystal sensor for remote bacteria population monitoring in raw milk via the Internet
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Series quartz crystal sensor for remote bacteria population monitoring in raw milk via the Internet

Ku-Shang Chang, Hung-Der Jang, Ching-Fu Lee, Yuan-Guey Lee, Chiun-Jye YuanSheng-Hsien Lee
Biosensors and Bioelectronics, 卷.21(8), 頁碼.1581-1590
02/2006
PMID: 16137878

摘要

Biosensor Escherichia coli Internet Series piezoelectric quartz crystal Biotechnology Biophysics Biomedical Engineering Electrochemistry
A remote monitoring system based on a piezoelectric quartz crystal (SPQC) sensor was developed for the determination of the bacteria population in raw milk. The system employs the Windows XP server operating system, and its programs for data acquisition, display and transmission were developed using the LabVIEW 7.1 programming language. The circuit design consists of a circuit with a piezoelectric quartz crystal (SPQC) and a pair of electrodes. This system can provide dynamic data monitoring on a web-page via the Internet. Immersion of the electrodes in a cell culture with bacteria inoculums resulted in a change of frequency caused by the impedance change due to microbial metabolism and the adherence of bacteria on the surface of the electrodes. The calibration curve of detection times against density of bacteria showed a linear correlation coefficient (R 2 = 0.9165) over the range of 70-10 6 CFU ml -1 . The sensor could acquire sufficient data rapidly (within 4 h) and thus enabled real-time monitoring of bacteria growth via the Internet. This system has potential application in the detection of bacteria concentration of milk at dairy farms. © 2005 Elsevier B.V. All rights reserved.

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