摘要
Two-dimensional (2D) transition metal dichalcogenides (TMDs) have gained immense research attention in biosensing due to their exceptional electrical and physical properties. However, the practical applications of these biosensors in point-of-care (POC) settings are hindered by the thermal instability of biological recognition elements, such as antibodies, which typically require meticulous storage conditions to retain their effectiveness. In this work, we develop a label-free molybdenum disulfide (MoS2) fieldeffect-based biosensor with enhanced thermal stability via silk fibroin encapsulation.Cardiac troponin I (cTnI), a key biomarker for acute myocardial infarction (AMI), was employed as a model analyte. The integration of a 2D MoS2 biosensor with silk fibroin provides a highly promising platform for sensitive and selective AMI diagnostics, achieving a remarkable limit of detection (LOD) of 1.14 pg/mL, significantly below clinical thresholds in human plasma. Furthermore, the resulting biosensors maintained high sensitivity (79.3% response) after storage at 40 °C for 1 week. This approach provides a potential solution for the development of 2D TMDs-based biosensors for practical applications.