摘要
A thermal lens microscope was applied to ultrasensitive detection of non-fluorescent molecule in liquid. The calibration curve showed good linearity in the 5.4-27 molecules region. The minimum number of molecules detected was 5.4 and the detection limit of Pb(II) octaethylporphyrin in benzene was 0.5 molecules. Our thermal lens microscope is a powerful tool for ultrasensitive detection of non-fluorescent molecules at the single-molecule level and offers a great potential for further applications. © 1999 Elsevier Science B.V. All rights reserved.