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Site-specific protein modification through CuI-catalyzed 1,2,3-triazole formation and its implementation in protein microarray fabrication
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Site-specific protein modification through CuI-catalyzed 1,2,3-triazole formation and its implementation in protein microarray fabrication

Po-Chiao Lin, Shau-Hua Ueng, Mei-Chun Tseng, Jia-Ling Ko, Kuo-Ting Huang, Sheng-Chieh Yu, Avijit Kumar Adak, Yu-Ju Chen 和 Chun-Cheng Lin
Angewandte Chemie - International Edition, 卷.45(26), 頁碼.4286-4290
06/2006
DOI: https://doi.org/10.1002/anie.200600756
PMID: 16739151

摘要

Copper Dimerization Ligation Nitrogen heterocycles Protein modifications Chemistry (all)
(Figure Presented) Out of site: A protein expression system was combined with Cu I -catalyzed 1,2,3-triazole formation to modify a target protein at its C terminus. The immobilized core protein can be modified by diverse small molecules in a site-specific manner (see picture). Protein conjugation with a diazido linker gives a homodimeric protein, and microarrays can be fabricated by site-specific covalent binding to functionalized surfaces. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

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