摘要
The development of efficient sensing element for rapid and sensitive measurement of glutathione is important on early detection of human diseases. Herein, we introduce the V 2 O 5 nanosheets (NS) as a novel nanozyme to biomimic the oxidase activity toward the rapid detection of glutathione. The 5–6 layered V 2 O 5 NS with 300–600 nm in size is successfully fabricated by ultra-sonication assisted exfoliation of bulk V 2 O 5 using N, N-dimethyl formamide as the intercalation reagent. The sensing mechanism involves the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by V 2 O 5 NS with the concomitant increase in absorption intensity. Upon introduction of glutathione, the oxidized TMB is converted back to its original form and results in the simultaneous decrease in peak intensity at 650 nm. The sensor exhibits a good linear response to glutathione in the range of 10–500 nM with a limit of detection of 2.4 nM. Moreover, the V 2 O 5 -TMB sensor exhibits a good selectivity and high specificity, which makes the detection of glutathione applicable in diluted human serum samples. Since no functionalization of the V 2 O 5 NS is required, this sensing system can open a new avenue to serve as an efficiently biomimic oxidase for glutathione detection in biological fluid samples.