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The effect of neuronal expression of heat shock proteins 26 and 27 on lifespan, neurodegeneration, and apoptosis in Drosophila
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The effect of neuronal expression of heat shock proteins 26 and 27 on lifespan, neurodegeneration, and apoptosis in Drosophila

Pin-Chao Liao, Hung-Yu Lin, Chiou-Hwa Yuh, Lin-Kwei YuHorng-Dar Wang
Biochemical and Biophysical Research Communications (BBRC), 卷.376(4), 頁碼.637-641
28/11/2008

摘要

Hsp26;Hsp27;Neuronal expression;Lifespan;Oxidative stress;hid-induced apoptosis;Polyglutamine toxicity;Parkinsonism climbing disorder
<p class="MsoNoSpacing"><span lang="EN-US" style="font-size:12.0pt"><span style="font-family:&quot;Calibri&quot;,&quot;sans-serif&quot;">Heat shock proteins (Hsps) are chaperones thought to increase lifespan, enhance stress resistance, and prevent apoptosis and neurodegenerative diseases. Our previous study reported that ubiquitous expression of hsp26&nbsp;or&nbsp;hsp27 extended<em> </em><em><span style="font-family:&quot;Calibri&quot;,&quot;sans-serif&quot;">Drosophila</span></em>&nbsp;lifespan. The effect of neuronal expression of hsp26 and hsp27 in Drosophila on the above-mentioned functions has not yet been investigated. Here, we show that neuronal expression of hsp26 and hsp27 improved lifespan and increased resistance to oxidative stress. However, only neuronal expression of hsp27 ameliorated Parkinsonism climbing disorder and attenuated mild polyglutamine-induced toxicity. Additionally, neuronal expression of hsp27 specifically partially rescued&nbsp;<em><span style="font-family:&quot;Calibri&quot;,&quot;sans-serif&quot;">hid-</span></em>induced lethality, but was not able to rescue <em><span style="font-family:&quot;Calibri&quot;,&quot;sans-serif&quot;">reaper/grim-</span></em>induced lethality. However, unlike&nbsp;<em><span style="font-family:&quot;Calibri&quot;,&quot;sans-serif&quot;">hsp27</span></em>, neuronal expression of hsp26 did not rescue&nbsp;<em><span style="font-family:&quot;Calibri&quot;,&quot;sans-serif&quot;">hid</span></em>-induced or&nbsp;<em><span style="font-family:&quot;Calibri&quot;,&quot;sans-serif&quot;">reaper/grim</span></em>-induced lethality. In summary, we demonstrate the functional similarities and differences of neuronal expression of&nbsp;<em><span style="font-family:&quot;Calibri&quot;,&quot;sans-serif&quot;">hsp26</span></em>&nbsp;and&nbsp;<em><span style="font-family:&quot;Calibri&quot;,&quot;sans-serif&quot;">hsp27</span></em>&nbsp;in adult&nbsp;<em><span style="font-family:&quot;Calibri&quot;,&quot;sans-serif&quot;">Drosophila</span></em>.</span></span></p>

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