Abstract
A positive myocardial inotropic effect achieved using HNO/NO - , compared with NO· triggered attempts to explore novel nitroxyl donors for use in clinical applications in vascular and myocardial pharmacology. To develop M-NO complexes for nitroxyl chemistry and biology, modulation of direct nitroxyl-transfer reactivity of dinitrosyl iron complexes (DNICs) is investigated in this study using a Fe III -porphyrin complex and proteins as a specific probe. Stable dinuclear {Fe(NO) 2 } 9 DNIC [Fe(μ- Me Pyr)(NO) 2 ] 2 was discovered as a potent nitroxyl donor for nitroxylation of Fe III -heme centers through an associative mechanism. Beyond the efficient nitroxyl transfer, transformation of DNICs into a chemical biology probe for nitroxyl and for pharmaceutical applications demands further efforts using in vitro/in vivo studies. A dinitrosyl iron complex, DNIC [Fe(μ- Me Pyr)(NO) 2 ] 2 , was found to be a potent nitroxyl donor for prospective clinical applications in vascular and myocardial pharmacology.