Abstract
The purpose of this study was to investigate the effect of supplementation of angelical sinensis diels, and glue of chineys reevesii plate and cervus nippon temmince horn (ACC) on oxidative damage induced by exhaustive exercise in rat erythrocytes. Twenty eight male Sprague-Dawley rats were randomly divided into the following three groups: 1) exhaustive exercise-drug(n=8), 2)exhaustive exercise (n= 10), and 3) control group (n=10). The exhaustive exercise started at 10% grade, 15 m/min for 15 min followed by gradual increases of treadmill speed and time until exhaustion. The rats in exhaustive exercise- drug group were supplemented ACC 5 g/kg/d for a week before exhaustive exercise. The running time to exhaustion in the exhaustive exercise-drug group(123.39 ± 17.98min) was significantly longer (p<.05) than that in the exhaustive exercise group (104.36 ± 33.64 min). Malondialdehyde (MDA) in the exhaustive exercised rat erythrocytes (1.77 ± 0.33 uM/L) was significantly higher than exhaustive exercise - drug (0.67 ± 0.42uM/L)and control group (0.42 ± 0.19 uM/L). In addition, deformability index (DI) of rat erythrocytes in exhaustive exercise group (0.52 ± 0.06) was markedly decreased than exhaustive exercise- drug (0.70 ± 0.05) and control group (0.74 ± 0.02)at 10th day after sacrifice. Moreover, scanning micrographic technique revealed that rat erythrocytes in exhaustive exercise group had hemolytic phenomenon. It is concluded that exhaustive exercise results in oxidative damage leading to a dramatic decrease in rat erythrocyte deformability and thus provided strong support for the hypothesis that free radical is a major factor affecting hemolytic anemia. And ACC could be one kind of antioxidants against oxidative damage induced by exhaustive exercise.