Abstract
A novel method, using a thermoreversible MC/PBS/Collagen hydrogel coated on the TCPS dish, for harvesting a living cell sheet or spheroid with ECM. In one application, the obtained living cell sheet/spheroid is administered to a joint adapted for implantation and for cartilage regeneration. In another application, the living cell sheet/spheroid is administered, preferably via percutaneous injection, to an infarcted cardiac tissue as a novel therapy for treating myocardial infarction.