Abstract
A method for bacterial genome editing includes: providing a bacterial cell; transforming a pCas9 plasmid and a pKD46 plasmid into the bacterial cell; co-transforming a pCRISPR::LacZ plasmid and an exogenous DNA into the bacterial cell carrying the pCas9 plasmid and the pKD46 plasmid, so as to obtain a strain broth; and spreading the strain broth on a culture medium to conduct cultivation.