Abstract
A Raman detecting chip for thin layer chromatography and a method for separating and detecting an analyte are provided. The Raman detecting chip for thin layer chromatography includes a silicon substrate. The silicon substrate includes a flat portion and a plurality of silicon nanowires disposed on the flat portion, wherein each silicon nanowire has a top surface and a sidewall. A metal layer covers the top surface and at least a part of the sidewall. The silicon nanowire has a length from 5 μm to 15 μm.