Abstract
A reverse transcription polymerase chain reaction (RT-PCR) chip is provided, which integrates a micro thermal control function in a micro fluid system so as to rapidly and automatically carry out a reverse transcription polymerase chain reaction to a virus specimen having a RNA base. The chip includes a base, a first reaction tank and a second reaction tank formed inside the base, a heating unit for heating the first and the second reaction tanks, a temperature sensing unit for measuring temperatures of the first and the second reaction tanks, and a pump unit for driving the specimen to flow from the first reaction tank to the second reaction tank. The specimen and a reverse transcription agent are filled in the first reaction tank to generate a reverse transcription reaction by controlling and heating through the heating unit and the temperature sensing unit. The pump unit drives partial complementary-DNA specimen through the reverse transcription reaction to flow to the second reaction tank, and the polymerase chain reaction agent is added to form a polymerase chain reaction by controlling and heating with the heating unit and the temperature sensing unit.