Abstract
The present invention provides a field-effect-transistor-based bio-sensor and the bio-signal amplification method thereof, which includes a FET chip, a bio-molecule fixation layer and at least one primer. The bio-molecule fixation layer is formed on the surface of gate electrode of FET chip or the surface of external component connected with the gate electrode. The primer used for the amplification of nucleotide is fixed to the surface of gate electrode of FET chip or the surface of external component through the formation of bonding with bio-molecule fixation layer, so that the object to be tested can have nucleotide amplification reaction with the primer in the room temperature or constant temperature. By extending the length of nucleotide sequence, the induced electricity on the surface of surface of FET gate can be increased, so that the detected signal is amplified, effectively enhancing the sensitivity of field-transistor-based biosensor.