Abstract
The invention relates to a gene editing system of Pseudomonas putida including a Pseudomonas putida, a RedCas expression plasmid and an exogenous gene expression plasmid. The RedCas expression plasmid includes a first replication origin, a first antibiotic resistance gene, a [lambda]-Red expression cassette and a Cas expression cassette. The exogenous gene expression plasmid includes a second replication origin, a left homology arm, a second antibiotic resistance gene, an exogenous gene expression cassette, a right homology arm and a gRNA cassette. The gene editing system of P. putida includes CRISPR system and [lambda]-Red system, which can homologously integrate of an exogenous gene into the polyploid chromosome of the P. putida to obtain a homologous recombinant with stable expression of the exogenous gene.