Abstract
A detection kit applied for detecting a body fluid sample of an organism. The detection kit includes a sampling device and a detecting device. The sampling device includes a sampling element and a filtering element. The filtering element is detachably disposed on the sampling element. The sampling element collects the body fluid sample filtered by the filtering element. The detecting device includes at least one reaction zone. The reaction zone includes a fiber substrate and a detection reagent. The detection reagent is immobilized on the fiber substrate. The reaction zone is configured to make the fiber substrate contact the body fluid sample collected by the sampling element. The fiber substrate absorbs the body fluid sample collected by the sampling element, thus making the body fluid sample collected by the sampling element react with the detection reagent for detection. The present invention further provides a detection method for detecting a body fluid sample of an organism. The present invention is advantageous for simplifying the pre-treatment process, small amount of reagent and rapid analysis.