Abstract
A detection structure including a substrate, a coating structure and a plurality of polyproline helix structure is provided. The substrate has a plurality of detecting regions. The coating structure is located on the substrate. The plurality of polyproline helix structure is located in each of the detecting region and on the coating structure. Each of the polyproline helix structure is composed of a plurality of proline monomers aligned in the first direction, aligned in the second direction and aligned in the third direction. The proline monomer aligned in the first direction is connected in the coating structure through a connecting structure, and the proline monomer aligned in the second direction is connected to at least two ligands. The two ligands on each of the polyproline helix structure has a fixed distance that can be appropriately controlled. A production method of the detection structure above is also described.