Abstract
This invention provides a biochip that may be applied in rapidly purifying deoxyribonucleic acid (DNA) and performing polymerase chain reaction (PCR), including a chip main body, and an electromagnetic adsorber, an electromagnetic temperature controller and a temperature sensor disposed upon the chip main body. The chip main body includes: four-sheet layers vertically overlapped and defining a mixing tank, two reaction tanks and a micro-channel; a two-way pump mechanism capable of controlling the flow direction of the liquid within the micro-channel; a vibration mechanism capable of driving the liquid in the mixing tank to generate turbulence; and two valve mechanisms capable of respectively plugging the micro-channel. With the structural design of the biochip, the entire DNA purification and PCR process are performed on the chip main body and the effect of extraction purification greater than the conventional extraction technique can be achieved. Moreover, with simple operating procedures and no need to use dangerous reagents, it is quite convenient and practical.