Abstract
The present invention provides an engineered cyanobacteria, comprising at least one expression plasmid-encoded three novel pathways to produce acetate, which can convert atmospheric carbon dioxide as a raw material into acetate. The present invention also construct the expression plasmid for three different transporters specific to acetate to be expressed in cyanobacteria, which comprises aspartate aminotransferase A (aatA), succinate-acetate/proton symporter (satP) and acid-tolerance P-type ATPase (actP). Therefore, the engineered cyanobacteria of the present invention can produce 0.58 mg/L to 3.54 mg/L of acetate per hour.