Abstract
A method is provided for producing 2,3-butanediol. An escherichia coli (E. coli) strain obtained through an innovation of genetic modification is used. In vivo of the strain, desired genes for producing 2,3-butanediol are expressed through gene transfer in a gene-recombination way. The present invention is characterized in that, during producing 2,3-butanediol, the strain does not change its yield owing to the change of oxygen concentration in the fermentation environment. The strain is capable of self-regulation by using an in-vivo metabolic pathway for dealing with the external environmental oxygen concentration. Thus, 2,3-butanediol is continuously produced with a high yield.