Abstract
The present invention relates to a microfluidic chip device for selecting a specific cell aptamer, comprising a plurality of storage reservoirs, a fluid control element, a reaction tank, and a polymerase chain reaction reservoir, wherein each storage reservoirs interconnect with each fluid control element via a pump/mixer, a sample and/or a reagent are mixed and further transmitted into each storage reservoirs. The present invention further provides a method for selecting specific cell aptamer via microfluidic chip device, comprising (a) providing a microfluidic chip device; (b) providing a plurality of nucleic acids (a nucleic acid library) and a plurality of samples (target cell), and mixing them; (c) purifying and rinsing the nucleic acid and the sample of step (b); (d) lysing the sample; (e) mixing a material of the sample of step (d) and a plurality of control cells; (f) purifying the material of step (e); and (g) proceeding polymerase chain reaction from the material of step (f) to obtain the cell aptamer.