Abstract
A chip for cell culture is disclosed, which comprises an air chamber forming plate, a channel forming plate, a forming plate of culture tank, and a substrate stacked upward sequentially. The channel forming plate has plural pump parts and valve parts located above the pump air chamber and the valve air chamber correspondingly. The forming plate of culture tank, in collaboration with the channel forming plate, defines a first channel for the culture solution to flow through the culture hole. When gas is pumped into the valve air chamber, the valve part can seal the bottom of the culture hole, so that the culture medium can be limited within the culture hole and condensed. When gas is pumped into the pump air chamber, the pump parts will protrude into the first channel respectively to squeeze the culture solution and push the culture solution to pass through the culture medium in the culture hole. Thus, the culture solution in the first channel can be refreshed automatically to save lots of labor and time, and to reduce the manual operation errors.