Abstract
A method for bacterial genome editing which include following steps: providing a bacterial host cell; introducing a pCas9 plasmid and a pKD46 plasmid into the bacterial host cell; introducing a pCRISPR::LacZ plasmid and a donor DNA into the pCas9 and pKD46 plasmids containing bacterial host cell, and obtaining a strain liquid; and spreading the strain liquid on an agar plate for strain cultivation.