Abstract
The invention relates to preparation of enterovirus 71 type virus-like particle by using a baculovirus recombination protein expression system and application of the virion. By modifying baculovirus vector design, hydrolysis protease 3CD expression is reduced and structural protein predecessor protein P1 is enhanced to increase virus-like particle (VLP) expression. The enterovirus virus-like particle can initiate high antibody titer reaction and neutralization titer reaction of mice, thereby indicating that the VLP can be used as vaccine of the enterovirus 71 type.