Abstract
The present invention is related to a method for preparing enterovirus 71 virus-like particle in the baculovirus/insect cell expression system and use thereof. By modifying the baculovirus vector design, the present invention lowers the expression level of protease 3CD and increases the expression level of the capsid protein precursor P1 so as to increase VLP yield. These VLPs can elicit high antibody titers and neutralization titers responses in mice, demonstrating to be as an enterovirus 71 vaccine.