Abstract
一種自組裝奈米粒子的製備方法，包含下述步驟。提供第一供體質體、第二供體質體及第三供體質體。進行質體轉位步驟。進行重組病毒製備步驟，以得第一重組桿狀病毒、第二重組桿狀病毒及第三重組桿狀病毒。進行轉導步驟，其係以第一重組桿狀病毒、第二重組桿狀病毒及第三重組桿狀病毒同時感染生產細胞，以使生產細胞表達核殼蛋白、包膜蛋白、工程化包膜蛋白及目標RNA，且核殼蛋白、包膜蛋白、工程化包膜蛋白及目標RNA係自組裝而得自組裝奈米粒子。藉此，可有效提升自組裝奈米粒子的生產與轉染效率，且自組裝奈米粒子具有優異的細胞靶向性。
A preparation method of self-assembling nanoparticle includes the following steps. A first donor plasmid, a second donor plasmid and a third donor plasmid are provided. A transposition step of plasmids is performed. A preparation step of recombinant viruses is performed so as to obtain a first recombinant baculovirus, a second recombinant baculovirus and a third recombinant baculovirus. A transduction step is performed, wherein a producer cell is simultaneously infected by the first recombinant baculovirus, the second recombinant baculovirus and the third recombinant baculovirus, and then a nucleocapsid protein, an envelope protein, an engineered envelope protein and a target RNA are expressed from the producer cell, and the nucleocapsid protein, the envelope protein, the engineered envelope protein and the target RNA self-assemble to form a self-assembling nanoparticle. Therefore, the production efficiency and the transfection efficiency of the self-assembling nanoparticle can be effectively enhanced, and the self-assembling nanoparticle can have excellent cell-targeting property.