Abstract
Reliable, scalable, and tunable SERS substrates are developed for quantitative SERS measurements and the limit of detection (LOD) can be down to single molecule level. This is achieved by the precise control of SERS enhancement factor and detection hot zone using ligand-regulated nanoparticle superlattices film with a built-in internal standard. The establishment of quantitative SERS technique will open up many exciting opportunities for both fundamental and applied research areas.