Abstract
An assay assembly includes an assay plate. The assay plate includes at least one hydrophobic area and a plurality of hydrophilic areas defined by the hydrophobic area, wherein the hydrophilic areas are defined as a plurality of assay areas. Due to the specialized structure of the assay plate, droplets around the assay areas are drawn to the assay area by pushing force of the hydrophobic area and the pulling force of the assay area. In addition, the shaker is coupled to the assay plate and configured for shaking the assay plate thereby droplets around the assay areas are further drawn to the assay area by shaking of the shaker. The present invention may improve the error-tolerance rate of the assay plate and be used for high-throughput screening.