專業
Laboratory Overview
Our laboratory focuses on advancing optical and histological methodologies for 3D characterization of tissue microarchitecture, vasculature, and innervation. By applying optical clearing methods, we render tissues—such as mouse and human intestine, pancreas, liver, and kidney—transparent, enabling detailed 3D visualization of fluorescently labeled structures. A key aspect of our research is integrating modern 3D histology (fluorescence and transmitted light imaging) with classic techniques, such as H&E and IHC staining, to enable multiplexed analysis of tissues in both health and disease.
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Antifade Solid-State Tissue Clearing for 3D & Super-Resolution Imaging
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Introduced by our laboratory in 2023, the antifade solid-state tissue clearing method is designed for 3D and super-resolution imaging, with demonstrated compatibility with 3D Airyscan, STED, Lattice SIM, and NSPARC. The method uses solid high-refractive-index (high-n) polymers to replace conventional immersion-based tissue clearing media.
Rather than using high-n liquids, we embed fluorescently labeled tissues in a solid, acrylamide-based high-n polymer through photo-polymerization. Much like tissue solidification with paraffin for H&E histology or resin for electron microscopy, this solvent-free environment is optimized for clinical tissue analysis.
By limiting oxygen diffusion into the specimen, it significantly reduces photobleaching and minimizes signal loss during imaging, while providing extended shelf life for long-term storage, transport, and repeated fluorescence imaging.
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Keywords: 3D histology; human pancreas early change/lesion; human pancreas & liver 3D neurohistology; high-n polymer, solid-state tissue clearing; antifade 3D & super-resolution imaging; 1080p HD video; 3D Airyscan; STED; Lattice SIM; NSPARC; 3D super-resolution islet imaging